Dual-regulated myoD- and msx1-based interventions in C2C12-derived cells enable precise myogenic/osteogenic/adipogenic lineage control
✍ Scribed by Cornelia Fux; Dominik Langer; Martin Fussenegger
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 463 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1099-498X
- DOI
- 10.1002/jgm.601
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Background
Advanced gene therapy, tissue engineering and biopharmaceutical manufacturing require sophisticated and well‐balanced multiregulated multigene interventions to reprogram desired mammalian cell phenotypes.
Methods
We have combined the streptogramin (PIP)‐ and tetracycline (TET)‐responsive gene regulation systems for independent expression control of the differentiation determinants myoD and msx1 in C2C12‐derived cells.
Results
Different dual‐regulated expression scenarios which induce either both, only one or none of the lineage control genes triggered differential differentiation and precise control of myogenic, osteogenic or adipogenic cell phenotypes.
Conclusions
Our findings substantiate the use of multiregulated multigene interventions in reprogramming cellular differentiation pathways in a desired manner. Copyright © 2004 John Wiley & Sons, Ltd.