Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart

✍ Scribed by Adrian Bradu; Lisha Ma; James W. Bloor; Adrian Podoleanu

• Book ID: 102871401
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 512 KB
• Volume: 2
• Category: Article
• ISSN: 1864-063X
• DOI: 10.1002/jbio.200910021

✦ Synopsis

Abstract

This article demonstrates a combined instrument of two imaging modalities to acquire information on cardiac function in larval Drosophila melanogaster: optical coherence tomography (OCT) and laser scanning fluorescence microscopy (LSFM). For this purpose, a dedicated imaging instrument able to sequentially provide cross‐sectional OCT and C‐scan LSFM images has been developed. With this dual‐imaging system, the heart can be easily located and visualized within the specimen and the change of the heart shape in a cardiac cycle can be monitored. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)