Dual effect of adenovirus-mediated transfer of BMP7 in mixed neuron-glial cultures: Neuroprotection and cellular differentiation
✍ Scribed by May-Jywan Tsai; Ching-Feng Weng; Song-Kun Shyue; Dann-Ying Liou; Chien-Hung Chen; Chuan-Wen Chiu; Tzu-Hsuan Yang; Hsu-An Pan; Roanna I-Hsin Liao; Huai-Sheng Kuo; Ming-Chao Huang; Wen-Cheng Huang; Barry J. Hoffer; Henrich Cheng
- Book ID
- 102383538
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 305 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Abstract
Bone morphogenetic proteins (BMPs), members of the TGF‐β superfamily, have been implicated in nervous system development and in response to injury. Previous studies have shown that recombinant BMP7 can enhance dendritic growth and protect cultured neurons from oxidative stress. Because of the presence of extracellular BMP antagonists, BMP7 seems to act locally. Therefore, the present study uses BMP7 overexpression using adenovirus (Ad)‐mediated gene transfer to examine its effect in mixed neuronal cultures. Enhanced BMP7 expression selectively induces neuronal CGRP expression in a time‐dependent manner. BMP7 overexpression not only significantly protects cultures from H~2~O~2~ toxicity but reduces lipopolysaccharide (LPS) stimulation. Concurrently, it profoundly reduces microglial numbers, but increases oligodendroglial and endothelial cells. Together, low‐dose and continuously expressed BMP7 is both neuroprotective and differentiation‐inductive. © 2007 Wiley‐Liss, Inc.