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Dual amino acid-selective and site-directed stable-isotope labeling of the human c-Ha-Ras protein by cell-free synthesis

✍ Scribed by Takashi Yabuki; Takanori Kigawa; Naoshi Dohmae; Koji Takio; Tohru Terada; Yutaka Ito; Ernest D. Laue; Jonathan A. Cooper; Masatsune Kainosho; Shigeyuki Yokoyama

Book ID: 110261158
Publisher: Springer Netherlands
Year: 1998
Tongue: English
Weight: 199 KB
Volume: 11
Category: Article
ISSN: 0925-2738
DOI: 10.1023/a:1008276001545

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For the application of multidimensional NMR spectroscopy to larger proteins, it would be useful to perform selective labeling of one of the 20 amino acids. For some amino acids, however, amino acid metabolism drastically reduces the efficiency and selectivity of labeling in in vivo expression system