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dsRNA-mediated gene silencing in cultured Drosophila cells: a tissue culture model for the analysis of RNA interference

✍ Scribed by Natasha J. Caplen; Jamie Fleenor; Andrew Fire; Richard A. Morgan


Book ID
117519905
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
444 KB
Volume
252
Category
Article
ISSN
0378-1119

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## Abstract RNA interference (RNAi) is a potent mechanism for local silencing of gene expression and can be used to study loss‐of‐function phenotypes in mammalian cells. We used RNAi to knockdown specifically the expression of choline acetyltransferase (ChAT), the enzyme of acetylcholine biosynthes