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Drosophilahemolymph proteins: Purification, characterization, and genetic mapping of larval serum protein 2 inD. melanogaster

✍ Scribed by Michael E. Akam; David B. Roberts; Jonathan Wolfe


Publisher
Springer
Year
1978
Tongue
English
Weight
908 KB
Volume
16
Category
Article
ISSN
0006-2928

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✦ Synopsis


Three of the major protein species present in the hemolymph of Drosophila melanogaster larvae just prior to pupation are absent from second instar larvae but accumulate rapidly during the third instar. This article describes the purification and characterization of one of these, larval serum protein (LSP) 2, using an immunological assay. It is a homohexamer of molecular weight about 450,000, with a polypeptide molecular weight of 78,000--83,000. Fast and slow electrophoretic variants of this protein map between the markers vin and gs, at 36--37 on chromosome 3.