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Droplet Digital™ PCR quantitation of HER2 expression in FFPE breast cancer samples

✍ Scribed by Heredia, Nicholas J.; Belgrader, Phillip; Wang, Shenglong; Koehler, Ryan; Regan, Jack; Cosman, Angela M.; Saxonov, Serge; Hindson, Benjamin; Tanner, Stephanie C.; Brown, Alexandra S.; Karlin-Neumann, George

Book ID
121267910
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
689 KB
Volume
59
Category
Article
ISSN
1046-2023

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A PCR assay using capillary electrophoresis was designed for the detection of c-erbB-2 gene amplification in alcoholformalin-acetic acid (AFA)-fixed, paraffin-embedded biopsies from 81 consecutive breast tumors. c-erbB-2 expression was analyzed in the same samples using immuno-histochemistry (IHC).