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DPS – A rapid method for genome sequencing of DNA-containing bacteriophages directly from a single plaque

✍ Scribed by Kot, Witold; Vogensen, Finn K.; Sørensen, Søren J.; Hansen, Lars H.

Book ID
122116263
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
679 KB
Volume
196
Category
Article
ISSN
0166-0934

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A polymerase chain reaction (PCR)-based technique is described which allows for the determination of library plasmid insert DNA sequence directly and rapidly from intact yeast cells. Yeast spheroplasts are used to template a PCR reaction to amplify the insert sequence. This PCR product is then purif