## Abstract **BACKGROUND:** Downstream processing plays a crucial role in biotechnological production processes. Product concentration and purification require complex, multi‐step operations, using significant amounts of chemical and auxiliary material. Today, design and optimization is important b
✦ LIBER ✦
Downstream of downstream processing – Integrated bioprocess development from upstream to downstream
✍ Scribed by F. Grote; R. Ditz; J. Strube
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- German
- Weight
- 77 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0009-286X
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## Abstract An integrated process strategy for the recovery of penicillin acylase was developed, based on precipitation of non‐enzymatic proteins directly from __Escherichia coli__ homogenates or crude extracts using Rolquat (quaternary ammonium salt) and adsorption of the enzyme on Amp‐Seph (3.8 µ