✦ LIBER ✦

Down-regulation of the nm23.h1 gene inhibits cell proliferation

✍ Scribed by Giovanna Cipollini; Andrea Berti; Lisa Fiore; Giuseppe Rainaldi; Fulvio Basolo; Generoso Bevilacqua; Maria A. Caligo

Publisher: John Wiley and Sons
Year: 1997
Tongue: French
Weight: 146 KB
Volume: 73
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19971009)73:2<297::aid-ijc22>3.0.co;2-b

No coin nor oath required. For personal study only.

✦ Synopsis

nm23 gene expression is strictly related to the state of cell growth. The level of its expression parallels the fraction of thymidine-incorporating cells (S-phase cells) in neoplastic mammary tissues and in the synchronously cycling fraction of MCF10A cells. nm23.h1 reaches a peak of expression in the S-phase, and is present at very low level during the G 0 /G 1 phase. Two strategies are used to demonstrate the direct involvement of the nm23.h1 gene in the process of cell proliferation. The first consists of transient inhibition of nm23.h1 expression by using anti-sense oligonucleotide treatment; weak inhibitory effect on cell proliferation is observed. The second strategy involves the stable inhibition of nm23.h1 expression by transfection of MCF10A cells with a plasmid vector expressing the human nm23.h1 anti-sense mRNA. The anti-sense-transfected cells show consistently slower proliferative activity than the control.

📜 SIMILAR VOLUMES

NM23 gene expression in human breast car

NM23 gene expression in human breast carcinomas: Loss of correlation with cell proliferation in the advanced phase of tumor progression

✍ Maria A. Caligo; Giovanna Cipollini; Andrea Berti; Paolo Viacava; Paola Collecch 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 378 KB 👁 1 views

NM23 is a protein associated with tumor progression, expressed in all tissues and in human tumors. Reduced expression of NM23.H1 is related to high incidence of lymph node and distant metastasis or to poor prognosis of the patient in several human malignant tumors. In this study we analyze NM23 expr

cAMP-mediated growth inhibition of lymph

cAMP-mediated growth inhibition of lymphoid cells in G1: rapid down-regulation of cyclin D3 at the level of translation

✍ Soheil Naderi; Kristine B. Gützkow; Jon Christoffersen; Erlend B. Smeland; Heidi 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 227 KB

Expression of a catalytically inactive H

Expression of a catalytically inactive H118Y mutant of nm23-H2 suppresses the metastatic potential of line IV Cl 1 human melanoma cells

✍ Carl V. Hamby; Rakesh Abbi; Nidhi Prasad; Charles Stauffer; Janet Thomson; Charm 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 130 KB

Differential expression of the neuroendo

Differential expression of the neuroendocrine genes Hel-N1 and HuD in small-cell lung carcinoma: Evidence for down-regulation of HuD in the variant phenotype

✍ Peter H. King 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 125 KB 👁 2 views

Hel-NI and HuD belong to the elav gene family and have gained recent attention as potential neuroendocrine markers for small-cell lung carcinoma (SCLC). Members of this conserved family normally appear at different stages of neuronal maturation, raising the possibility that their expression patterns