Double-stranded regions in denatured DNA from mouse cells

✍ Scribed by Robert B. Church; Georgii P. Georgiev

• Publisher: Springer
• Year: 1973
• Tongue: English
• Weight: 291 KB
• Volume: 1
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00357401

✦ Synopsis

Approximately 270 of the DNA of the mouse genome reassociates at infinitely low Co t values, 10 -7 to 10 -6 moles 1-1 s. The melting profile of the reassociation product, which is resistant to nuclease S~ digestion, has been characterized by hydroxyapatite column chromatography. The properties of these nuclease resistant sequences suggest that they exist as 'DNAhairpins' and that they originate from reverted base sequences within the genome.