Double-spot formation in the thin-layer chromatography of pure fenfluramine hydrochloride and some other amines in the presence of organic solvents

✍ Scribed by B. Wesley-Hadzija

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 587 KB
• Volume: 79
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85294-8

✦ Synopsis

Prom a number of solutions of fenfluramine hydrochloride in (a) ethanol or (b) chloroform, chromatographed on thin layers of silica gel or alumina, using the solvent systems chloroform-methanol (I : I), methanol-acetone (r : I) and chloroform-ethanol (4: I), two spots were produced, whereas solutions in water gave only one spot. This phenomenon did not occur when the sulfate salts of the amines were used. Similar results were obtained with amphetamine and ethylamphetamine hydrochlorides. The material extracted from the faster moving spots was identified as fenfluramine hydrochloride and that from the lower spots as fenfluramine. Partial and complete hydrolysis, depending on the ionic strength of the acid, were factors leading to the formation of double spots (ethanolic solutions) and single spots (aqueous solutions) of the amine hydrochlorides.