Double Quantum Filtered 1H NMR Spectroscopy Enables Quantification of Lactate in Muscle

✍ Scribed by I. Asllani; E. Shankland; T. Pratum; M. Kushmerick

• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 100 KB
• Volume: 152
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.2001.2407

✦ Synopsis

In this study we address the question of quantification of muscle lactate using double quantum filtered (DQF) 1 H NMR spectroscopy where dipolar and scalar coupled spectra are acquired. For this, lactate content in muscle samples was independently determined using a conventional enzymatic assay and DQF, 1 H NMR spectroscopy. NMR quantification of lactate relied on comparison of muscle spectra with similarly acquired spectra of standard lactate solutions. Transverse relaxation, T 2 , and dipolar coupling effects were investigated at two different orientations of muscle fibers relative to B o and at various lactate concentrations. In all cases, we found a biexponential T 2 decay of the lactate methyl signal with a long T 2 of 142 ms (±8 ms, n = 24) and a short T 2 of 37 ms (±6 ms, n = 24). Lactate content of muscle determined by NMR spectroscopy agreed with the results obtained from enzymatic assays of the same samples provided that T 2 effects as well as the presence of both scalar and dipolar coupling interactions of lactate in muscle were taken into account.