Double-Label Simultaneous Time-Resolved Fluoroimmunoassay of Phenytoin and Phenobarbital

✍ Scribed by Chang Wen-Bao; Zhang Bai-Lin; Li Yuan-Zong; Ci Yun-Xiang

• Book ID: 102617312
• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 113 KB
• Volume: 55
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1996.1305

✦ Synopsis

A procedure for the simultaneous time-resolved fluoroimmunoassay for phenobarbital and phenytoin, based on the use of europium-and samarium-labeled haptens, has been investigated. These lanthanide ions are bound to the haptens by means of the anhydride of diethylenetriaminepentaacetic acid. The antibody is immobilized onto immunoplate in which samples are assayed in competitive immunoassay. After the immunoreactions and dissociation with a fluorescence enhancement solution, the fluorescence intensity is measured. The detection limits of the assay are, respectively, 20 ng/ml for phenobarbital and 50 ng/ml for phenytoin. Results obtained with the proposed methods correlate well (CV õ10%). The inter-reactions between phenobarbital, phenytoin, and their antibodies are studied.