✦ LIBER ✦
Double isotopic method using dansyl chloride for the determination of GABA in rat C6 astrocytoma cell cultures
✍ Scribed by R.L. Kohl; W.B. Quay; J.R. Perez-Polo
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 321 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Methods are described for the quantitative measurement of GABA in culture. The method can be adapted to any amino acid or dansyl-chloride-reactive species. The sensitivity and selectivity of the procedure result from the double isotopic design in which (14C)-labeled internal standard was added to the samples before reaction with (3M)-labeled dansyl chloride. Values obtained by ion-exchange amino acid analysis of cultures agree closely with the values obtained by the double isotopic method. This method is sensitive enough to measure GABA intracellularly and the condition medium.