𝔖 Bobbio Scriptorium
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Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures

✍ Scribed by Françoise Lasne


Book ID
114051294
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
132 KB
Volume
276
Category
Article
ISSN
0022-1759

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Partition of free and monoclonal-antibod
✍ L. Elling; M.-R. Kula; E. Hadas; E. Katchalski-Katzir 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 415 KB

The principle that the antigen and the antibody prefer different phases in an aqueous two-phase system is the analytical basis of the work presented here. The antigen horseradish peroxidase, which is bound to a monoclonal antibody (mAb), is separated from free Ag in an aqueous phase system (polyethy