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Dot-immunobinding assay in the detection of IgG antibodies against farmer's lung antigens

✍ Scribed by Rauno M. Mäntyjärvi; Viswanath P. Kurup

Publisher
Springer Netherlands
Year
1988
Tongue
English
Weight
883 KB
Volume
103
Category
Article
ISSN
0301-486X

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✦ Synopsis

Circulating antibodies against Faenia rectivirgula, Thermoactinomyces candidus, T. vulgaris and Aspergillus fumigatus were studied in the sera of 14 clinically proven farmer's lung patients and 10 normal controls using three immunological methods. These methods were agar gel double diffusion (DD), biotin-avidin-linked immunosorbent assay (BALISA) and dot-immunobinding assay (DIBA). Agar gel diffusion, the least sensitive of the three methods, failed to detect antibodies in some of the patients, while BALISA detected antibodies even in the normal controls. However, the sensitivity of dot-immunobinding assay was in between DD and BALISA while the specificity was comparable to DD to all the antibodies except against A. fumigatus antigens. Dot-immunobinding assay gave faster results than DD and the blots can be stored as recordfor longer periods of time without fading.

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