✦ LIBER ✦

Dot-blot assays and their use as a direct antigen-binding method to screen monoclonal antibodies to 1,4-β-and 1,3-β-glucan synthases

✍ Scribed by Patrice Nodet; Jacques Grange; Michel Fevre

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 316 KB
Volume: 174
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90070-x

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid method has been developed to assay &&can synthases spotted on a nitrocellulose sheet. The sensitivity of this method allows screening of hybridoma-making monoclonal antibodies in a direct antigen-binding assay by measurement of the activity of the enzymes retained by the antibodies previously fixed on nitrocellulose. 0 1988 Academic Press, IIIC.