Dose-response relationships for hepatic aflatoxin B1-DNA adduct formation in the rat in vivo and in vitro: The use of immunoslot blotting for adduct quantitation
✍ Scribed by J.C. Phillips; S. Davies; B.G. Lake
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 106 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0270-3211
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✦ Synopsis
An immunoslot blotting (ISB) method for quantitating aflatoxin B1-DNA adduct levels has been developed and used to examine the relationship between dose and hepatic aflatoxin B1-DNA adduct levels in rats fed aflatoxin B1 (AFB1) in the diet at dose levels of between 0.5 and 10 micrograms/kg/day. The method has also been used to examine the dose-response relationship for adduct formation in precision-cut rat liver slices incubated with AFB1 at concentrations between 0.01 and 2 microM. For the feeding studies, groups of male Fisher F344 rats were given AFB1 in the diet for periods of 1 to 10 weeks and hepatic DNA adduct levels determined using ISB. The time for adduct levels to reach steady-state conditions was determined in animals given approximately 10 micrograms of AFB1/kg/day and steady-state levels at lower concentrations measured. The time course for the accumulation of AFB1-DNA adducts in rat liver slices incubated with AFB1 at 0.5 microM has been investigated and the relationship between adduct formation and AFB1 concentration over a wide concentration range in liver slices has been determined.