Dominant-negative p53 can restore tumorigenicity of L-929 cells in immunocompetent mice
✍ Scribed by Leonard J. Appleman; Alan B. Frey
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- French
- Weight
- 987 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
To study the effect of a transforming allele of the tumor suppressor p53 upon the anti-tumor immune response, antigenic L-929 cells were transfected with the dominant-negative valinel3' mutant of murine p53. Several p53v~~~~5-expressing transfectants formed non-regressing tumors in immunocompetent hosts. The growth rates of tumorigenic and non-tumorigenic clones were equivalent in vitro in sublethally irradiated C3H/HeN mice and in nude mice. Tumorigenic and nontumorigenic p53"I1 35-expressing L-929 clones expressed equivalent levels of cell surface class I major histocompatibility complex (MHC) glycoproteins. Immunization with a tumorigenic clone protected mice from subsequent challenge and primed MHC class I-restricted cytotoxic T-lymphocytic precursors. Secretion of an immunosuppressive cytokine, transforming growth factor p-I and sensitivity to tumor necrosis factor-a