Dominant lethal induction by ethyl methanesulfonate in the male axolotl (Ambystoma mexicanum)

✍ Scribed by Armstrong, John B. ;Gillespie, Laura L.

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 582 KB
• Volume: 212
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402120314

✦ Synopsis

Abstract

When male axolotls (Ambystoma mexicanum) were treated with ethyl methanesulfonate (EMS) and mated at regular intervals thereafter, the incidence of embryonic abnormalities among the F~1~ progeny increased until a time was reached when none survived to hatching. At 100 mg/1 EMS, this point was reached about 130 days after treatment. Thereafter, the frequency of abnormalities gradually decreased to control levels. At higher concentrations, abnormalities were seen in spawnings obtained sooner after treatment, and also at earlier developmental stages. The pattern is similar to that reported for the mouse, which has been attributed to differential sensitivity of the various germ cell stages to the mutagenic agent. The time course, however, is greatly extended in the axolotl. In future experiments we will be looking for gene mutations, primarily before and after the period of peak mortality.