Dominant expression of sex-hormone-binding-globulin exon-7 splicing variant over wild-type mRNA in human ovarian cancers
✍ Scribed by Ryou Misao; Yoshihito Nakanishi; Jiro Fujimoto; Shigenori Iwagaki; Teruhiko Tamaya
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- French
- Weight
- 97 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
The intracellular expression of sex-hormone-bindingglobulin(SHBG) exon-7-splicing-variant mRNA in human ovarian cancers was demonstrated by reverse-transcription/ polymerase-chain-reaction, Southern-blot and DNAsequencing analyses. Analysis of the missing base pairs proved that they corresponded to the entire exon 7, which is considered to encode a portion of the steroid-binding site, suggesting that the steroid-binding affinity of this variant might be different from that of the SHBG wild type. SHBG wild-type and variant mRNA was detected in all normal ovaries and in benign and malignant ovarian tumors analyzed. There were no significant differences in mean SHBG wildtype and variant mRNA levels among the 3 types of tissue, but the ratio of SHBG exon-7-splicing-variant to wild-type mRNA level in ovarian cancers was significantly higher (p F 0.05) than that in normal ovaries, with over-expression in some benign tumors. SHBG mRNA levels and the ratio of SHBG variant to wild-type mRNA was not associated with histological classification or clinical stages of ovarian cancers. These results suggest that over-expression of SHBG-exon 7-splicing-variant mRNA to the wild type might indicate the potential for neoplastic transformation.