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Does the antitumor cyclopropylpyrroloindole antibiotic CC-1065 cross-link DNA in tumor cells?

โœ Scribed by Andrzej Skladanowski; Marcin Koba; Jerzy Konopa


Book ID
113992501
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
191 KB
Volume
61
Category
Article
ISSN
0006-2952

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Until now the fluorescence assay with ethidium bromide has only been used on pure DNA. This assay depends on the difference in fluorescence between single-and double-stranded D N A (dsDNA). Cross-links in D N A are measured by the return of fluorescence of dsDNA after heat denaturation at PH 12. Und