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Does F1-ATPase have a catalytic site that preferentially binds MgADP?

โœ Scribed by Hui Z. Mao; Wesley D. Gray; Joachim Weber


Book ID
116466804
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
256 KB
Volume
580
Category
Article
ISSN
0014-5793

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ATP hydrolyses by the wild-type a&y and mutant (aD261N)3P3y subcomplexes of the FI-ATPase from the thermophilic Bacillus PS3 have been compared. The wild-type complex hydrolyzes 50 p M ATP in three kinetic phases: a burst decelerates to an intermediate phase, which then gradually accelerates to a fi