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Docosahexaenoic acid decreases phospholipase A2 activity in the neurites/nerve growth cones of PC12 cells

✍ Scribed by Rex E. Martin

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
199 KB
Volume
54
Category
Article
ISSN
0360-4012

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✦ Synopsis

Docosahexaenoic acid (DHA) accumulates in nerve growth cones (NGC) during perinatal development and it is neuroprotective in ischemia. Because the phospholipases A 2 (PLA 2 ) are present in NGC and these enzymes function in both ischemia and longterm potentiation, the relationship between DHA and PLA 2 was investigated in the NGC of nerve growth factor-differentiated PC12 cells. When PC12 cells were incubated with [ 3 H]DHA, it primarily esterified in ethanolamine glycerolipids and concentrated initially in cell bodies with similar levels present in the neurite/nerve growth cone (N/NGC) fraction after 4 days. PLA 2 activity in the N/NGC fraction was investigated using [ 14 C]arachidonic acid-labeled phosphatidylinositol ([ 14 C-AA]PI) as substrate. Heat denaturation and pharmacological inhibition showed that much of the PLA 2 activity was calcium-independent and secretory rather than cytosolic. Supplementing the media with as little as 33 nM DHA significantly reduced PLA 2 activity in the N/NGC fraction.

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