Cells of Tetrahymena pyriformis have been cold-synchronized using a repetitive cycle of six, two-hour cold shocks (9.5"C) alternating with decreasing periods (60-30 minutes.) at 28Β°C. This system gives a maximum division index of 7040% occurring at 90 minutes from the end of the last synchronizing c
β¦ LIBER β¦
Do Synchronizing Temperature Shifts Inhibit RNA Synthesis in Tetrahymena pyriformis?1
β Scribed by BYFIELD, JOHN E. ;LEE, YOUNG C.
- Book ID
- 109246605
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 1970
- Tongue
- English
- Weight
- 1017 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0022-3921
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## Abstract Nuclei were isolated by exposing temperature synchronized __Tetrahymena pyriformis__ (HSM) to TritonβXβ100. Cell division synchrony was induced with a repetitive 12βhour temperature cycle (9.5 hours at 13Β°, 2.5 hours at 29Β°). Increase in nucleic acid content was biphasic: primarily duri