Do static or time-varying magnetic fields in magnetic resonance imaging (3.0 T) alter protein–gene expression?—A study on human embryonic lung fibroblasts
✍ Scribed by Nina F. Schwenzer; Rüdiger Bantleon; Brigitte Maurer; Rainer Kehlbach; Christina Schraml; Claus D. Claussen; Enno Rodegerdts
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 137 KB
- Volume
- 26
- Category
- Article
- ISSN
- 1053-1807
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Purpose
To evaluate the influence of magnetic resonance imaging (MRI) on gene expression in embryonic human lung fibroblasts (Hel 299).
Materials and Methods
The cells were exposed to the static magnetic field and to a turbo spin‐echo sequence of an MR scanner at 3.0 Tesla. An MR group (exposed) and a control group (sham‐exposed) were set up using a special MR‐compatible incubation system. The exposure time was two hours. Gene expression profiles were studied using a complementary deoxyribonucleic acid (cDNA) microarray containing 498 known genes involved in transcription, intracellular transport, structure/junction/adhesion or extracellular matrix, signaling, host defense, energetics, metabolism, cell shape, and death.
Results
No changes in gene expression were found in either group (exposed or sham‐exposed cells) at the end of a two‐hour exposure for any of the 498 tested protein genes.
Conclusion
The results suggest that MRI has no influence on protein–gene expression in eugenic human lung cells. J. Magn. Reson. Imaging 2007;26:1210–1215. © 2007 Wiley‐Liss, Inc.