Do leukocytes contribute to impaired microvascular tissue perfusion after arterial repair?
✍ Scribed by Frank-W. Peter; Dale A. Schuschke; Wei Z. Wang; Gary L. Anderson; Ralph J.P.M. Franken; Silvia Pierangeli; John H. Barker
- Book ID
- 101273526
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 218 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0738-1085
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✦ Synopsis
Impaired capillary perfusion may result in flap failure. Platelet emboli, polymorphonuclear leukocytes (PMNs), and/or vasospasm have been identified as possible causes. This study investigates the role of PMNs in causing impaired capillary perfusion in a free flap model. PMN concentrations were depleted using antineutrophil serum. The cremaster muscles of 20 Sprague-Dawley rats were isolated on a single neurovascular pedicle and after a simulated technically poor arterial anastomosis upstream and reperfusion, capillary perfusion was measured each hour for 6 hours. Even though the number of PMNs was significantly reduced in the animals treated with antineutrophil serum, capillary perfusion was not changed compared with controls. These results demonstrate that depleting circulating PMNs does not protect capillary perfusion in our model. These findings suggest that reduced capillary perfusion downstream from an anastomotic repair is not mediated by the presence of PMNs in the microcirculation.