DNA vaccination of mice with plasmid expressing human papillomavirus 6 major capsid protein L1 elicits type-specific antibodies neutralizing pseudovirions constructed in vitro
✍ Scribed by Matsumoto, Koji; Kawana, Kei; Yoshikawa, Hiroyuki; Taketani, Yuji; Yoshiike, Kunito; Kanda, Tadahito
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 104 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
Human papillomavirus 6 (HPV 6) causes benign condylomata. As a model for HPV vaccine development, we tested a HPV 6 DNA vaccine candidate, constructed by subcloning the major capsid protein (L1) gene into an expression plasmid having the cytomegalovirus promoter, for its immunogenicity in BALB/c mice. Three intracutaneous inoculations of the plasmid with a gene gun at 2-week intervals elicited anti-L1 serum antibodies. The antibodies were found to recognize highly type-specific, conformation-dependent epitopes, including those to neutralize pseudovirions capable of inducing -galactosidase in infected monkey COS-1 cells. The data support the idea that immunization with DNA capable of expressing HPV L1 can be used as an HPV vaccine strategy for humans.