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DNA typing of HLA-A using polymerase chain reaction (PCR) and sequence-specific oligonucleotide probes (SSOP)


Book ID
116114331
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
93 KB
Volume
44
Category
Article
ISSN
0198-8859

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A simple and rapid method for characterizing the polymorphism at the HLA-DPB1 locus has been developed. The procedure involves the selective amplification of the polymorphic second exon of the DPB1 locus by the polymerase chain reaction (PCR), followed by hybridization of the amplified DNA with 15 n