DNA — Technology and Its Forensic Application

✍ Scribed by A. J. Jeffreys, N. J. Royle, I. Patel, J. A. L. Armour, A. MacLeod, A. Collick (auth.), Prof. Dr. rer. biol. hum. G. Berghaus, Prof. Dr. med. B. Brinkmann, Prof. Dr. med. C. Rittner, Prof. Dr. med. M. Staak (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1991
• Tongue: English
• Leaves: 225
• Edition: 1
• Category: Library

✦ Synopsis

All up-to-date aspects of DNA technology are discussed partly in review lectures but mostly in research articles in this volume: new methods, population statistics for different restriction fragment length polymorphisms (RFLP's), new developments dealing with the polymerase chain reaction (PCR), biostatistical aspects of single locus and multi locus profiles as well as examples of practical applications in paternity testing and forensic stain analysis. Contributors to this volume include most internationally acclaimed researchers in this field. Besides facts that are primarily of interest to forensic scientists, immunohaematologists and human geneticists should also find some aspects for their research.

✦ Table of Contents

Front Matter....Pages I-XI
Front Matter....Pages 1-1
Principles and Recent Advances in DNA Fingerprinting....Pages 3-19
DNA Fingerprinting as a Tool of Paternity Testing in Germany....Pages 20-32
Genetic Typing of Biological Evidence Using the Polymerase Chain Reaction....Pages 33-40
A Critical Review of the Forensic Application of DNA Technology....Pages 41-49
DNA Methylation: A Defense Mechanism Against the Expression of Foreign DNA?....Pages 50-58
Juristische Aspekte der forensischen DNA-Analyse....Pages 59-67
Front Matter....Pages 69-69
Research Regarding the Polymerase Chain Reaction for Forensic Utility — HLA-DQα Locus and AMP-FLPs....Pages 71-78
DNA Typing Using PCR Amplified Fragments in the HLA Class I Region....Pages 79-84
PCR-Typing of the Human HLA-DQα Locus: Population Genetics and Application in Forensic Casework....Pages 85-91
DNA Amplification (PCR) of Several Hypervariable Loci in Disputed Paternity Cases....Pages 92-95
Amplification of a Highly Polymorphic VNTR Segment, Localized on the Ha-Ras Gene by PCR....Pages 96-100
Front Matter....Pages 101-101
Reliability of Restriction Enzyme Digestions of Genomic DNA for the Generation of DNA Fingerprints....Pages 103-108
Concept for a More Precise Definition of the Polymorphism YNH24....Pages 109-115
Evaluation of Variable Number of Tandem Repeat (VNTR) Alleles in Mother-Child Combinations....Pages 116-120
A Proposed Combination of Single Locus Probes for Multiple Rehybridization....Pages 121-127
DNA-Profiles with HVR-Probes — Allele Frequencies of HinfI-RFLPs Using pHINS310, pMUC7 and pMR24/1 in a Population Sample from Hannover and Lower Saxony....Pages 128-133
Analysis of Allele Frequencies of Two Polymorphic Loci in a North-East Italian Population....Pages 134-140
Digoxigenin-DNA Probes for Detecting Human VNTR Polymorphism....Pages 141-144
Front Matter....Pages 145-145
The Application of Four Single Locus Probes in Cases of Disputed Paternity....Pages 147-152
The Practical Significance of Human Genetic RFLP-Systems in Paternity Testing....Pages 153-156
Front Matter....Pages 145-145
Individual Diagnostics by DNA Fingerprinting....Pages 157-159
Paternity Analysis in a Deficient Family by DNA Profiling (Single-Locus and Multi-Locus Probes)....Pages 160-164
Paternity Tests in 10–12 Weeks Old Fetuses by Means of Single-Locus-DNA-Polymorphisms....Pages 165-169
DNA Fingerprinting for the Protection of Endangered Species....Pages 170-171
Front Matter....Pages 173-173
Treatment of Bloodstains for Simultaneous Application of Serological and DNA Typing Methods....Pages 175-180
DNA Extraction and RFLP Analysis of Bloodstains on a Variety of Textiles — Investigation of Various Extraction Procedures....Pages 181-186
Stability of DNA in Brain Cortex After Long Post Mortem Periods....Pages 187-191
The Identification of a Charred Body by Oligonucleotide Fingerprinting with the (GTG) 5 Probe....Pages 192-195
Front Matter....Pages 197-197
Biostatistical Evaluation of Evidence from Single Locus Hypervariable DNA Probes: Tests of Independence of Loci....Pages 199-202
MLP and SLP Band Patterns and Probabilities of Identity....Pages 203-207
Efficiency of Multilocus Probes in Mother-Child-Putative Father Cases....Pages 208-211
The Proposal of Evett et al. for Paternity Calculations in Normal Trio Cases Using MLP Profiles and the Freiburg Model of a Multiple Diallelism: A Comparison....Pages 212-220
A Contribution to the Computer-Aided Analysis of DNA Fingerprint Patterns....Pages 221-225

✦ Subjects

Forensic Medicine; Human Genetics; Hematology; Statistics for Life Sciences, Medicine, Health Sciences