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DNA Synthesis Catalyzed in vitro by Yeast Extracts

✍ Scribed by Gianfranco BADARACCO; Enrico GINELLI; Nicoletta SACCHI; Silvio SORA; Paolo PLEVANI


Book ID
115120816
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
828 KB
Volume
118
Category
Article
ISSN
1432-1327

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DNA synthesis catalyzed in vitro by yeas
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Partially purified cell-free extracts prepared from growing cells of the yeast Saccharomyces cerevisiae catalyze DNA synthesis directed by the chimeric plasmid BTYP-2 containing the whole 2 ΞΌm DNA sequence. The biochemical properties of the reaction have been examined and inactivation studies indica

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✍ Johnston, Leland H. πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 447 KB

A system is described in which DNA synthesis can be monitored in a yeast lysate. The observed synthesis has many of the properties of in vivo DNA replication. It is dependent upon replication growing points that were active in vivo. The in vitro synthesis proceeds via low molecular weight intermedia