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DNA synthesis by isolated nuclei of Physarum polycephalum

✍ Scribed by E.N. Brewer; H.P. Rusch

Book ID: 118849048
Publisher: Elsevier Science
Year: 1965
Tongue: English
Weight: 289 KB
Volume: 21
Category: Article
ISSN: 0006-291X
DOI: 10.1016/0006-291x(65)90277-9

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## Abstract Homogenates of __Physarum polycephalum__ incorporate [^3^H] dATP into nuclear DNA at an initial rate of approximately 15% of the in vivo rate. To attain this level of synthesis, cultures are homogenized in a medium containing Mg^++^, EGTA, glucose and spermine. Incorporation is strongly