DNA structure in the nucleoprotein complex that activates replication of phage Ø/29

In vitro initiation of bacteriophage Φ29

In vitro initiation of bacteriophage Φ29 and M2 DNA replication: Genes required for formation of a complex between the terminal protein and 5′dAMP

✍ Matsumoto, Kouji ;Saito, Toshiyuki ;Hirokawa, Hideo 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 966 KB

Cell-free extracts prepared from phi 29 and M2-infected Bacillus subtilis cells catalyse the formation of complexes between terminal protein and [alpha-32P]-dAMP in the presence of [alpha-32P]-dATP, MgCl2, ATP, and phage DNA with terminal protein covalently linked at both the 5'ends. The complex for