Molecular cloning of heterologous chromo
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Gryczan, Thomas ;Contente, Sara ;Dubnau, David
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Article
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1980
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Springer
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English
β 926 KB
The difficulty experienced in the shotgun cloning of chromosomal DNA on plasmid vectors in Bacillus subtilis is analyzed and an explanation for this difficulty is offered based on an inherent property of competent cells which imposes a requirement of plasmid multimers in transformation of plasmid-fr