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DNA sequencing of the Escherichia coli ribonuclease III gene and its mutations

✍ Scribed by Nashimoto, Hiroko ;Uchida, Hisao

Publisher: Springer
Year: 1985
Tongue: English
Weight: 525 KB
Volume: 201
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00397981

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✦ Synopsis

A 0.7 kb DNA fragment of the Escherichia coli K12 chromosome was shown to contain the structural gene for RNAse III (rnc). The DNA sequence of the gene was determined and its alteration in an RNAse III defective mutant, AB301-105, was identified. DNA sequence analysis also showed that a secondary-site suppressor of a temperature-sensitive mutation in the E. coli ribosomal protein gene, rpsL, occurred within the rnc gene, providing genetic evidence for the interaction of ribosomal proteins with RNAse III, which in turn acts on the nascent ribosomal RNA during assembly of ribosomes in E. coli.

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