𝔖 Bobbio Scriptorium
✦   LIBER   ✦

DNA sequence and functional analysis of an ARS-element from the zygomyceteAbsidia glauca

✍ Scribed by Anke Burmester; Johannes Wöstemeyer

Book ID
104743384
Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
463 KB
Volume
12
Category
Article
ISSN
0172-8083

No coin nor oath required. For personal study only.

✦ Synopsis

A DNA fragment of mitochondrial origin from the mucoraceous fungus Absidia glauca promoting autonomous plasmid replication in Saccharomyces cerevisiae was sequenced and functionally characterised. We could show that the original mitochondrial insert cloned in Yip5 contains two regions with ARS activity which mutually inhibit each other. All plasmid derivatives replicating in yeast are rapidly lost during growth under nonselective conditions. In addition to one ARS consensus sequence with only one base substitution, the mitochondrial insert contains 18 related sequences with two base pair exchanges. With one exception all consensus sequences are preceded by sequence motifs strongly resembling ARS boxes.

📜 SIMILAR VOLUMES

An autonomously replicating sequence fro
An autonomously replicating sequence from HeLa DNA shows a similar organization to the yeast ARS1 element
✍ Valenzuela, Manuel S. 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 793 KB

A HeLa DNA fragment, which may function as an anchorage point to the nuclear matrix for human chromosomes 1 and 2, also functions as an autonomously replicating sequence (ARS) in the yeast Saccharomyces eerevisiae. In the present report we show that this DNA fragment contains both bent DNA and an A-