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DNA replication patterns of the early S phase from amniotic fluid cells as revealed by a Giemsa staining technique

✍ Scribed by J. T. Epplen; T. Bauknecht; W. Vogel

Publisher: Springer
Year: 1976
Tongue: English
Weight: 153 KB
Volume: 31
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00270408

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✦ Synopsis

We report on DNA replication patterns of the early S phase demonstrated by a BUdR/Hoechst (33258)-fluorescence/Giemsa technique. Replication starts in R bands and spreads within 1 hr over the whole chromosome complement with exceptions concerning heterochromatin. The beginning and the duration of replication are discussed.

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The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques.--Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type