DNA-repeat instability is associated with colorectal cancers presenting minimal chromosome rearrangements
✍ Scribed by Yorghos Remvikos; Nicolas Vogt; Martine Muleris; Rémy J. Salmon; Bernard Malfoy; Bernard Dutrillaux
- Book ID
- 102222042
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 470 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
The DNA-repeat [ (CA)n] instability of colorectal cancer cells was studied relative to our previously defined classification based on chromosome alterations. Of the 23 tumors analyzed, 13 belonged to the "monosomic" type (MT) characterized by simultaneous loss of chromosome I8 and chromosome arm I7p, and many structural rearrangements, 7 to the "trisomic" type (TT) with many chromosome gains but few rearrangements, and 3 had a normal karyotype (NT). (CA)n repeat sequences were examined on chromosomes 2 , 5 , I I, 13, 18, and 20. We found sequence alterations in I 2 tumors at I or several loci, 9 of which (1/13 MT, 5/7 TI-, and 3/3 NT) exhibited a typical shift in allele size defined as microsatellite instability. Furthermore, a single alteration was observed for the MT tumor, whereas one NT tumor displayed instability on two and all the other tumors on three or more loci. These results suggest an inverse relationship between the occurrence of chromosome structural rearrangements and microsatellite instability, providing another argument for the subdivision of colorectal cancers into groups of distinct oncogenic pathways. Genes Chromosom Cancer 12:272-276 (1995). 0 I995 Wiley-Liss, Inc.