Content:
Assays for Determining Lesion Bypass Efficiency and Mutagenicity of Site‐Specific DNA Lesions In Vivo Review Article
Pages 1-15
James C. Delaney, John M. Essigmann
Oxidative DNA Glycosylases: Recipes from Cloning to Characterization Review Article
Pages 15-33
Viswanath Bandaru, Jeffrey O. Blaisdell, Susan S. Wallace
Purification and Characterization of NEIL1 and NEIL2, Members of a Distinct Family of Mammalian DNA Glycosylases for Repair of Oxidized Bases Review Article
Pages 33-48
Tapas K. Hazra, Sankar Mitra
Analysis of Base Excision DNA Repair of the Oxidative Lesion 2‐Deoxyribonolactone and the Formation of DNA–Protein Cross‐Links Review Article
Pages 48-64
Jung‐Suk Sung, Bruce Demple
Isolation and Analyses of MutY Homologs (MYH) Review Article
Pages 64-78
A‐Lien Lu‐Chang
Use of Yeast for Detection of Endogenous Abasic Lesions, Their Source, and Their Repair Review Article
Pages 79-91
Serge Boiteux, Marie Guillet
Activities and Mechanism of DNA Polymerase ОІ Review Article
Pages 91-107
William A. Beard, Rajendra Prasad, Samuel H. Wilson
Direct Removal of Alkylation Damage from DNA by AlkB and Related DNA Dioxygenases Review Article
Pages 108-120
Barbara Sedgwick, Peter Robins, Tomas Lindahl
Purification and Characterization of DNA Photolyases Review Article
Pages 121-156
Gwendolyn B. Sancar, Aziz Sancar
Genetic and In Vitro Assays of DNA Deamination Review Article
Pages 156-170
Heather A. Coker, Hugh D. Morgan, Svend K. Petersen‐Mahrt
The Xeroderma Pigmentosum Group C Protein Complex and Ultraviolet‐Damaged DNA‐Binding Protein: Functional Assays for Damage Recognition Factors Involved in Global Genome Repair Review Article
Pages 171-188
Kaoru Sugasawa
Purification and Characterization of Escherichia coli and Human Nucleotide Excision Repair Enzyme Systems Review Article
Pages 189-213
Joyce T. Reardon, Aziz Sancar
Assays for Transcription Elongation by RNA Polymerase II Using Oligo(dC)‐Tailed Template with Single DNA Damage Review Article
Pages 214-223
Isao Kuraoka, Kiyoji Tanaka
In Vivo Assays for Transcription‐Coupled Repair Review Article
Pages 223-246
Graciela Spivak, Gerd P. Pfeifer, Philip Hanawalt
TFIIH Enzymatic Activities in Transcription and Nucleotide Excision Repair Review Article
Pages 246-263
Jean‐Philippe Lainé, Vincent Mocquet, Jean‐Marc Egly
An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts Review Article
Pages 264-273
James Reid, Jesper Q. Svejstrup
Analysis of the Excision Step in Human DNA Mismatch Repair Review Article
Pages 273-284
Jochen Genschel, Paul Modrich
Characterization of the “Mismatch Repairosome” and Its Role in the Processing of Modified Nucleosides In Vitro Review Article
Pages 285-303
Katja Baerenfaller, Franziska Fischer, Josef Jiricny
Analysis of DNA Mismatch Repair in Cellular Response to DNA Damage Review Article
Pages 303-317
Liya Gu, Guo‐Min Li
Characterization of Escherichia coli Translesion Synthesis Polymerases and Their Accessory Factors Review Article
Pages 318-340
Penny J. Beuning, Sharotka M. Simon, Veronica G. Godoy, Daniel F. Jarosz, Graham C. Walker
Measuring the Fidelity of Translesion DNA Synthesis Review Article
Pages 341-355
Scott D. McCulloch, Thomas A. Kunkel
DNA Polymerases for Translesion DNA Synthesis: Enzyme Purification and Mouse Models for Studying Their Function Review Article
Pages 355-378
Paula L. Fischhaber, Lisa D. McDaniel, Errol C. Friedberg
Purification and Characterization of Escherichia coli DNA Polymerase V Review Article
Pages 378-390
Katharina Schlacher, Qingfei Jiang, Roger Woodgate, Myron F. Goodman
Yeast and Human Translesion DNA Synthesis Polymerases: Expression, Purification, and Biochemical Characterization Review Article
Pages 390-407
Robert E. Johnson, Louise Prakash, Satya Prakash
Localization of Y‐Family Polymerases and the DNA Polymerase Switch in Mammalian Cells Review Article
Pages 407-415
Patricia Kannouche, Alan Lehmann
Repair of DNA Double Strand Breaks: In Vivo Biochemistry Review Article
Pages 416-429
Neal Sugawara, James E. Haber
Assays for Nonhomologous End Joining in Extracts Review Article
Pages 430-444
Joe Budman, Gilbert Chu
Purification and Assays of Saccharomyces cerevisiae Homologous Recombination Proteins Review Article
Pages 445-463
Stephen Van Komen, Margaret Macris, Michael G. Sehorn, Patrick Sung
Analysis of DNA Recombination and Repair Proteins in Living Cells by Photobleaching Microscopy Review Article
Pages 463-485
Jeroen Essers, Adriaan B. Houtsmuller, Roland Kanaar
Synthetic Junctions as Tools to Identify and Characterize Holliday Junction Resolvases Review Article
Pages 485-501
Ulrich Rass, Stephen C. West
In Vitro Nonhomologous DNA End Joining System Review Article
Pages 502-510
Yunmei Ma, Michael R. Lieber
RAG and HMGB1 Proteins: Purification and Biochemical Analysis of Recombination Signal Complexes Review Article
Pages 511-528
Serge Bergeron, Dirk K. Anderson, Patrick C. Swanson
Purification and Biochemical Characterization of Ataxia‐Telangiectasia Mutated and Mre11/Rad50/Nbs1 Review Article
Pages 529-539
Ji‐Hoon Lee, Tanya T. Paull
Series Editors
Page ii
Contributors to Volume 408
Pages ix-xii
Methods in Enzymology
Pages xiii-xxxv
Author Index
Pages 541-567
Subject Index
Pages 569-582
Table of Contents
Pages v-vii