DNA profiles and numeric histogram classifiers in nephrogenic adenoma

✍ Scribed by Wiener, Helene G. ;Remkes, Gerard W. ;Birner, Peter ;Pycha, Armin ;Schatzl, Georg ;Susani, Martin ;Breitenecker, Gerhard

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 124 KB
• Volume: 96
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/cncr.10475

✦ Synopsis

BACKGROUND.

The malignant potential of nephrogenic adenoma is still a matter of controversy and therapeutic regimens of this morphologic entity range from partial, even total cystectomy to watchful waiting. The objective of the current study was to evaluate several robust image cytometry-DNA histogram classifiers and to search among those for factors that separate a biologically nonaggressive metaplastic lesion from lesions with increased malignant potential.

METHODS.

The study included bladder irrigation specimens, 23 preceding transurethral resection of nephrogenic adenoma and 24 preceding resection of papillary bladder carcinoma. Feulgen-stained nuclei were imported to a static image analysis system, and densitometric data were interpreted by two different software programs. Histograms were described numerically by DNA index, 2c deviation index, and by 5c/9c-exceeding and euploid polyploidy rates. In addition, an interpretation algorithm based on a dual parameter analysis with an integrated automatic threshold was used.