DNA polymorphism analysis in five endangered species of Meconopsis (Himalayan poppy) using multi-copy sequence-based probes
✍ Scribed by Irshad M. Sulaiman; Dr. Seyed E. Hasnain
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 453 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
DNA polymorphism analysis in five endangered species of Meconopsis (Himalayan poppy) using multi-copy
Eukaryotic Gene Expression sequence-based probes
Laboratory, National Institute of Immunology, New Delhi
An attempt to understand the genetic basis of extinction of Meconopsis (Himalayan poppy), an endangered genus of ornamental value and confined to alpine Himalayas, is described. Multicopy DNA sequences were isolated from a Sau3A partial genomic plasmid library of M. simplicifolia and M. paniculata. Genetic polymorphism between five species of Meconopsis belonging to geographically isolated populations was evident, albeit at a low level, in genomic Southerns when probed with repetitive DNA clones isolated from this library. Intra-population monomorphic band patterns for different populations of a species was evident with respect to a number of enzymes suggesting very low or no genetic variability and/or low mutation rate within a given population of Meco n ops is.