DNA-ploidy studies on cytological preparations from breast cancers by image analysis: Comparison with feulgen staining performed in destained Papanicolaou slides
✍ Scribed by Angelo Sidoni; Antonio Cavaliere; Patrizia Alunno; Emilio Bucciarelli
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 366 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
Image cytometric analysis of DNA ploidy status of 40 fresh primary breast carcinomas was performed in a comparative study on cytologic preparations stained directly by the Feulgen method and smears from the same tumors Feulgen-stained after Papanicolaou staining. There was a 0.82 overall concordance in diploid and aneuploid tumor classification and a good correlation (r = 0.736, P -= 0.001 1 between the DNA indices determined by the two methods. Discordances are probably due to tumor heterogeneity. This study demonstrates that image cytometric DNA analysis on previously routine-stained cytologic preparations is feasible and reliable for pretreatment DNA analysis and suggests potential applications in retrospective studies with cytologic material from archives. o is96 Witey-Liss, tnc.