DNA mutagenesis and repair in UV-irradiated E. coli K-12 under condition of mutation frequency decline
β Scribed by Anna Fabisiewicz; Celina Janion
- Book ID
- 114071476
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 478 KB
- Volume
- 402
- Category
- Article
- ISSN
- 0027-5107
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The mutation recL152 leads to a reduction of excision repair as measured by an increase in the time required to close uvrA uvrB dependent incision breaks, and by a reduction of host cell reactivation ability. Postreplication repair is also delayed when measured in a uvrB5 recL152 double mutant. Such
Toluene treated cells have been used to study the processes of DNA synthesis and DNA degradation in ultra-violet irradiated Escherichia coli K12. Synthesis and degradation are both shown to occur extensively if polynucleotide ligase is inhibited, and to occur to a much lesser extent if ligase activi