DNA Methylation: Methods and Protocols

✍ Scribed by Jörg Tost (auth.), Jörg Tost (eds.)

• Publisher: Humana Press
• Year: 2009
• Tongue: English
• Leaves: 407
• Series: Methods in Molecular Biology 507
• Edition: 2
• Category: Library

✦ Synopsis

Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.

✦ Table of Contents

Front Matter....Pages i-xvi
Front Matter....Pages 1-1
Front Matter....Pages 3-20
Front Matter....Pages 21-21
Front Matter....Pages 23-34
Front Matter....Pages 35-41
Front Matter....Pages 43-43
Back Matter....Pages 45-54
....Pages 55-64

✦ Subjects

Human Genetics; Gene Expression