DNA methylation in the glial fibrillary acidic protein gene: Map of CpG methylation sites and summary of analysis by restriction enzymes and by LMPCR
✍ Scribed by B. Teter; C. E. Finch; D. F. Condorelli
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 167 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
report the methylation status of CpG sites in the rat GFAP gene in various tissues and cells. This summary of these independent investigations describes the CpG sites evaluated and the correspondence of their results based on two different techniques for the evaluation of DNA methylation, i.e., methylation-sensitive restriction endonucleases , and genomic sequencing combined with LMPCR .
The relative advantages and disadvantages of the two techniques for the evaluation of methylation levels at CpG sites are described in detail in the respective papers. Briefly, restriction enzyme analysis evaluates a subset of all CpG sites over a wide genomic region, but is limited in its ability to resolve close-lying sites; LMPCR analysis quantitatively evaluates absolute methylation levels at all CpG sites within a short region (-200 bp for one PCR primer set).
The CpG sites evaluated so far in the GFAP gene are shown in Figure . In particular, two CpG sites were evaluated by both methods in the upstream promoter at -1170 and in exon 1 at + 115 (these sites are reported as -1176 and + 114 in the differ-