DNA Methylation Changes in the IL-I (2F) Chromosomal Region of Some Radiation-Induced Acute Myeloid Leukaemias Carrying Chromosome 2 Rearrangements
✍ Scribed by Andrew Silver; Andrew George; Walter Masson; George Breckon; Julie Adam; Roger Cox
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 841 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Acute myeloid leukaemias (AML) arising in irradiated CBA/H mice frequently have breakpoints in the F region of chromosome 2. The closely linked cytokine genes interleukin (/L)-/a and p map to this region, and the p gene is deregulated in some AMLs. Using pulsed-field gel electrophoresis techniques, we show here that an 800 kb 2F region encoding /L-/ a and p is not obviously rearranged in six leukaemias carrying chromosome 2 abnormalities. However, changes in /L-/ region DNA methylation in three leukaemias may be consistent with loss of hypermethylated sequences from one chromosome copy. These possible 2F region losses are discussed in relation t o genomic imprinting and its potential role in murine myeloid leukaemogenesis.