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DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions

✍ Scribed by Jerry M. Orosz; James G. Wetmur

Publisher
Wiley (John Wiley & Sons)
Year
1977
Tongue
English
Weight
816 KB
Volume
16
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

DNA melting temperatures and renaturation rates have been determined for Me~2~Et~2~NBr and a series of RMe~3~NBr and REt~3~NBr solvents where R is a linear hydrocarbon chain. The point of independence of DNA melting temperature on base composition has been investigated for each solvent system. Renaturation rates are compared with those found in other concentrated salt solutions. Solvent mixtures which accelerate DNA renaturation have also been investigated.

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