✦ LIBER ✦
DNA-ligand interaction detected by proton selective and non-selective spin-lattice relaxation rate analysis
✍ Scribed by Claudia Bonechi; Alessandro Donati; Maria Pia Picchi; Claudio Rossi; Enzo Tiezzi
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 423 KB
- Volume
- 115
- Category
- Article
- ISSN
- 0927-7757
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✦ Synopsis
In this paper, a method based on NMR measurements for studying the ability of ligands to interact with macromolecule surfaces is proposed. It is based on temperature-dependent NMR proton selective and non-selective spin lattice relaxation rate experiments and assumes fast chemical exchange conditions of the ligand between free and bound states. The results obtained appear able to identify DNA-psoralen complex formation. The use of this melhodology for monitoring non-covalent interaction of ligands and bipolymers is suggested.