DNA image cytometry on sections as compared with image cytometry on smears and flow cytometry in melanoma

✍ Scribed by T. Klapperstück; W. Wohlrab

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 837 KB
• Volume: 25
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/(sici)1097-0320(19960901)25:1<82::aid-cyto9>3.0.co;2-g

✦ Synopsis

DNA measurements of 130 melanomas were carried out by flow cytometry (FCM) and image cytometry (ICM). ICM was applied to cytological preparations of fresh material (cICM) and to sections of formalin-hxed parallh embedded tissue (sICM). The DNA ploidy, the DNA index of GO/Gl peaks (DI), and the proliferation index (PI) were used to compare all the methods. The following parameters reflecting malignancy were calculated only from ICM histograms: the 5c exceeding rate (ScER) and the In cases found to be DNA aneuploid by FCM, the PI values (FCM versus cICM) and the DIs (between all methods) showed a high correlation, and the concordance in relation to the DNA ploidy status was 96% (FCM versus cICM) and 94% (FCM versus sICM). However, we ascertained essential differences between FCM and ICM in melanomas classi-malignancy grade (MG).

fied as DNA diploid by FCM. The concordance in DNA ploidy was only 66% (FCM versus cICM) and